Quant-iT PicoGreen dsDNA Reagent. 反应次数. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 5). 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Instead of 2 mL, only 1 mL measurement volume was used. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. Unit Size. Picogreen assays are compatible with most microplate readers and fluorometers. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. The accuracy index is defined in Table 2. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quantitation Range. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. UV흡광 결과보다 민감도 우수. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic. Price from $9. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). 2023. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. high-range. BR reagent (Component A) 1. 50 pg to 2 µg. PicoGreen dsDNA quantitation reagents are. 12. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The PicoGreen is sticking to the sides of the cuvette or assay plate. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. g. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). 1X dsDNA assay (%) 100 17. The assay is highly selective for double- stranded DNA over RNA, and in the. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. AccuBlue® RNA Quantitation Kit Details. 50 pg to 2 µg. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. low-range. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 995 mL TE for the . ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Catalog Number. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. 0 fluorometer and Quant-iT PicoGreen dsDNA. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. Figure 1. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. Picogreen assays are compatible with most microplate readers and fluorometers. , QuantIt PicoGreen dsDNA assay). Rapidly detect as little as 0. • Suitable for use in 96- and 384-well plate formats. • Suitable for use in 96- and 384-well plate formats. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. 2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. Materials: 1. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. 2023. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. 3. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. According to the obtained results, the assay presents a linear correlation in a wide range of 0. a) 16. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. • Specific for dsDNA in the presence of RNA. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Product Type. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. Determination of DNA Yield. • Specific for dsDNA in the presence of RNA. 5 Q32851 15. Picogreen assays are compatible with most microplate readers and fluorometers. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. Learn how to use the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit to quantitate double-stranded DNA (dsDNA) in solution with a fluorometer or a microplate reader. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. no. 2 – 100 ng. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. 99 to $1999. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. Pico488 allows to quantitate as little as low pg/mL of dsDNA. Picogreen assay kit (Invitrogen, P7589) a. . 50 ul PicoGreen Mix by swirling bottle. Description. 5. Showing 4 of 4. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. to each well. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. 激发/发射. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as. This kit contains. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. • Suitable for use in 96- and 384-well plate formats. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. The normalized DNA was aliquoted into multiple tubes at 50µL volume. 10pg/µl vs. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. were treated with standard-of-care chemotherapy in vitro followed by cfDNA quantification via a PicoGreen dsDNA assay. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. ” application on Lunatic and compared to Qubit and. Rapidly detect as little as 0. PicoGreen dsDNA Assay Kit. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. methods for the quantitation of DNA: the Invitrogen™ Quant-iT™ PicoGreen™ dsDNA assay and the Invitrogen™ Qubit™ dsDNA assays. 99. samples as well as standard in the assay itself. Quantification and normalization of double stranded DNA with minimal set-up time. 5 µL of each extracted sample was used for Picogreen analysis. Picogreen assays are compatible with most microplate readers and fluorometers. • Specific for dsDNA in the presence of RNA. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. 5 ng/µL. Picogreen assays are compatible with most microplate readers and fluorometers. Libraries were sequenced on the Illumina NovaSeq. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. This protocol is a simplified and condensed. Thermo Fisher quant it picogreen dsdna quantitation assay kit. Picogreen assays are compatible with most microplate readers and fluorometers. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Rapidly detect as little as 0. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. High tolerance for contaminants. No. 21 DiB) [22]. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. Rapidly detect as little as 0. Quantify nucleic acids and proteins using four user-selectable fluorescent. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Picogreen assays are compatible with most microplate readers and fluorometers. Table 2 summarizes results of the. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Quant-iT dsDNA BR buffer (Component B) 250 ml c. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. USA) and quantity was measured using Qubit 2. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. Dilute from 20xTE stock (supplied in the PicoGreen kit). Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). • Specific for dsDNA in the presence of RNA. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. 2. • Suitable for use in 96- and 384-well plate formats. Bioz Stars score: 86/100, based on 1 PubMed citations. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. 5mL of TE x 5uL PicoReagent. Price from $9. The four reactions per sample were pooled, cleaned up with 1x AMPureXP beads, resuspended in 30 ul EB (10 mM Tris-HCl, pH 8. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. Selectively detect dsDNA from a wide variety of sources, and even in presence of. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). 10. stranded DNA (dsDNA). Quant-iT RiboGreen RNA Reagent The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. Volume of 20xTE = final volume/20;Application tip demonstrating that with Molecular Devices SpectraMax® microplate textbooks and the Quant-iT PicoGreen assay, users can reliably measure contents as low when 50 pg/mL of double-stranded DNA. Figure 2. 0 ml 200x in DMSO b. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Description. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. Qubit RNA XR Standard can be used with either Qubit RNA XR Assay. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Facebook. 9 Q33230 9 500 28. (2012). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). This protocol is a simplified and c. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). • Specific for dsDNA in the presence of RNA. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. The libraries were sequenced using the Illumina HiSeq 2500. VIEWS. 2022. This protocol is a simplified and condensed version of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. a)Use 110 sample number per plate. 0. クイックオーダー. 2. 95 mL TE for the . Highly selective for dsDNA over ssDNA or RNA. Quant-iT RNA BR (Broad Range) Assay Kit. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quantitation Range. Rapidly detect as little as 0. The Rapidly detect as little as 0. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. Table 3. 995 mL TE for the . Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Fluorometer. . 2–100 ng, the fluorescence signal is linear with DNA. • Suitable for use in 96- and 384-well plate formats. 2. The PicoGreen reagent was discarded and the cells were rinsed with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) solution for 10 min. Picogreen assays are compatible with most microplate readers and fluorometers. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Product Type. 125 to 100 ng/mL. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. 3,201 3. n = 3. 326,933 327K. 522. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. • Specific for dsDNA in the presence of RNA. Average 86 stars, based on 1. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. Bioz Stars score: 99/100, based on 1 PubMed citations. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Table 2 summarizes results of the. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. 2. PicoGreen dsDNA quantitation reagents are. 微孔板读数仪. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. Picogreen assays are compatible with most microplate readers and fluorometers. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. 86100 stars. 1 mL kit. 2023-11. Picogreen assays are compatible with most microplate readers and fluorometers. 2–100 ng: 2-1,000 ng: 0. Picogreen assays are compatible with most microplate readers and fluorometers. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Picogreen assays are compatible with most microplate readers and fluorometers. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). Katalognummer: P11495. 3. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Rapidly detect as little as 0. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Specific for dsDNA in the presence of RNA. 2–100 ng of DNA. Mix PicoGreen and DNA by pippetting up and down 5-10 times. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. 2–100 ng, the fluorescence signal is linear with DNA. 3. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Rapidly detect as little as 0. 86. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. • Specific for dsDNA in the presence of RNA. Calibrator MPO. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 9. Q33130). P11496). Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. Catalog Number. No. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. The assay is linear over three orders of magnitude and has little sequence. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. • Suitable for use in 96- and 384-well plate formats. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. For duplicate-well droplet. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 2–100 ng Quant-iT dsDNA HS Assay Kit 0. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Qubit DNA and RNA assays are available with different quantification ranges. This sensitivity exceeds that achieved with the Hoechst 33258. 50 pg to 2 µg.